LEGIONELLA SAMPLING GUIDEwww.legionellatesting.com
1. Personal safety and precautions should be observed during sampling. Avoid breathing aerosols that may be contaminated with Legionella bacteria. Avoid generating aerosols or water mists during sampling of the water system. Turn off the cooling tower fan during sampling, or wear a respirator equipped with a HEPA cartridge.
2. Prepare or obtain sterile, screw-capped plastic bottles for sampling. Obtain an extra bottle as a field blank. Sodium thiosulfate is routinely added to the bottle as a preservative and halogen (chlorine)-neutralizing agent. A 1 liter water sample is recommended for potable water sampling and 250 ml for non-potable water sampling.
3. For drinking or potable water, such as water fountains, faucets, and shower heads, collect two samples if possible. Collect the "pre-flush or first draw" sample by draining the first 1000 ml of water from the faucets or flush drains into a bottle. Allow the water to run for approximately 60 seconds (or longer) and collect the second draw of 1000 ml of water. Leave a one-inch space on top of the water sample. The second sample is also called "post-flush or second draw" sample.
4. When sampling faucet aerators and showerheads, swabs of faucet aerators and inside the faucet and shower heads should be taken. When sampling cooling towers, whirlpool spas or fountains, look for areas of biofilm and take a swab sample of the biofilm. We will provide sterile swabs for this purpose.
5. For non-drinking or non-potable samples from such sources as cooling towers, chillers, condensate pans, surface water in reservoirs, sprinklers, etc., collect 250 ml water from the bottom or side of the vessel or reservoir. If taking a cooling tower sample, also consider taking a sample in the pack column. Leave a one inch space on top of the sample. Record any biocide used in water treatment when collecting non-drinking water. If sampling whirlpool spas, consider taking a swab sample of any biofilm as well as a sample of the sand filter.
6. Label sample number on the bottle and record on the sample data sheet. Use a distinctive number for each sample. Complete all sample information on a sample data sheet for your own record. Send a copy with the samples to the laboratory.
7. Tightly cap the bottles. Make sure that water does not leak during shipping and transporting. Taping of bottle around the cap and neck with electric vinyl tape is recommended. Place taped bottles in a clean plastic bag.
Place the samples in insulated boxes or on freezer packs to protect specimens from extreme temperature fluctuations in the summer months. NEVER USE ICE OR DRY ICE. Stuff the box with foam chips to cushion, and seal the box securely for shipping. Send samples by overnight express carrier. Schedule sampling between Monday and Friday so that samples can be delivered to the laboratory no later than Saturday. Take holidays into consideration.
Please address the mailer as follows for Saturday delivery (use our normal address as listed above for Monday thru Friday delivery):
EMSL Analytical Inc.
18 Roland Ave
Mt. Laurel , NJ 08054
HOLD AT FEDEX LOCATION
Free sterile, preserved bottles and swabs
Broad Screen Legionella analysis (36 species as Spp.) with a 2 day turnaround by PCR (M163)
Identificaton and Enumeration of Legionella- 10 species plus serotyping of L. pneumophila (M212)
Identification and Enumeration of L. pneumophila serotypes 1-14 (M211)
Presumptive Enumeration of Legionella species (M210)