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Subject Food Authenticity & Contamination Identification
Sandwich sample wrapped in a napkin was sent to the lab for DNA analysis for maggot or insect materials contamination. The DNA analysis samples were taken from the cheese, egg, meat and bread around the bite mark. There were "white granules" spread in the burger that were included in DNA analysis. DNA was extracted using Tissue Extraction kit on an automatic DNA extraction instrument. DNA was analyzed for standard DNA barcode for mitochondrial COI (cytochrome c oxidase I) gene as well as universal DNA mini-barcode for COI.

The results showed that the cheese was from cow, the meat was from pork. The egg did not produce any amplifiable DNA and no DNA sequence available. The bread had three types of DNA materials including wheat (Triticum aestivum), cow (Bos taurus) and beetle DNA (Ochthebius minimus, Dendroacalles anagaensis or Prosimulium arvum). Although universal DNA mini-barcode could not reproduce beetle DNA sequences due to quantity of the trace amount of beetle DNA, it is still possible that the sandwich was contaminated with beetle larva (such as mealworm), instead of "maggot (fly larvae)". In addition, the sandwich bread and meat was dissected under microscope and no "maggot" like larvae or worms were observed, suggesting the beetle larva perhaps only presented on the surface.
Ann Arbor, MI - NVLAP Lab Code 101048-4Atlanta, GA - NVLAP Lab Code 101048-1Baton Rouge, LA - NVLAP Lab Code 200375-0Beltsville, MD - NVLAP Lab Code 200293-0Boston, MA - NVLAP Lab Code 101147-0Buffalo, NY - NVLAP Lab Code 200056-0Calgary, Alberta - NVLAP Lab Code 500100-0Carle Place, NY - NVLAP Lab Code 101048-10Charlotte, NC - NVLAP Lab Code 200841-0Chicago, IL - NVLAP Lab Code 200399-0Corporate - Cinnaminson, NJ - NVLAP Lab Code 101048-0Dallas, TX - NVLAP Lab Code 600111-0Denver, CO - NVLAP Lab Code 200828-0EMSL Canada - Montreal -- Quebec - NVLAP Lab Code 201052-0EMSL Canada -- Toronto - NVLAP Lab Code 200877-0EMSL Canada Inc. -- Ottawa - NVLAP Lab Code 201040-0Fort Lauderdale - NVLAP Lab Code 500085-0Houston, TX - NVLAP Lab Code 102106-0Huntington Beach, CA - NVLAP Lab Code 101384-0Indianapolis, IN - NVLAP Lab Code 200188-0Inland Empire, CA - NVLAP Lab Code 600239-0Kernersville, NC - NVLAP Lab Code 102104-0Las Vegas, NV - NVLAP Lab Code 600140-0Miami, FL - NVLAP Lab Code 200204-0Minneapolis, MN - NVLAP Lab Code 200019-0New York, NY - NVLAP Lab Code 101048-9Orlando, FL - NVLAP Lab Code 101151-0Pasadena, CA - NVLAP Lab Code 200232-0Phoenix, AZ - NVLAP Lab Code 200811-0Piscataway, NJ - NVLAP Lab Code 101048-2Plymouth Meeting, PA - NVLAP Lab Code 200699-0Raleigh, NC - NVLAP Lab Code 200671-0Rochester, NY - NVLAP Lab Code 600183-0S. Portland, ME - NVLAP Lab Code 500094-0Salem, NH - NVLAP Lab Code 201051-0San Diego, CA - NVLAP Lab Code 200855-0San Leandro, CA - NVLAP Lab Code 101048-3Seattle, WA - NVLAP Lab Code 200613-0St. Louis, MO - NVLAP Lab Code 200742-0Tampa, FL - NVLAP Lab Code 600215-0Vancouver, BC - NVLAP Lab Code 201068-0Wallingford, CT - NVLAP Lab Code 200700-0West Palm Beach, FL - NVLAP Lab Code 600206-0Weymouth, MA - NVLAP Lab Code 600217-0
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